Can a capillary electrophoresis system produce an accurate sized electropherogram?

This study describes an alternative approach using a capillary electrophoresis (CE) system to produce an accurately sized electropherogram. A variety of patient samples were examined, including solid tissue, peripheral blood, bone marrow aspirates, and paraffin-embedded tissue.

How many dNTPs are in a 7 L PCR reaction?

The PCR reactions were performed in 7 μL reaction mixture containing 4.32 μL PCR water, 0.66 μL Taq Buffer KCl-MgCl2 (10X), 0.51 μL MgCl 2 (25 mM), 0.33 μL dNTPs (2.5 mM), 0.06 μL forward/reverse primer (12.5 μM each), 0.06 μL Maximo Taq DNA Polymerase (5 U/μL) (GeneON, Deutschland, Germany), and 1 μL DNA (25 ng/μL).

Can dyads be categorized based on page-derived genomic data?

However, relatedness inferred from each dataset showed that the categorization of dyads in the different relationship categories strongly differed. This suggests that while scientists can reliably use PAGE-derived genomic data to estimate genetic diversity, they cannot use the same for parentage testing.