Is there a dye-based staining method for DNA in polyacrylamide gels?

Results: A fast and sensitive visible dye-based staining method for DNA in polyacrylamide gels using basic fuchsin (BF) is described.

Can online polyacrylamide gel electrophoresis be used for nucleic acid detection?

We developed an online polyacrylamide gel electrophoresis platform for nucleic acid detection. The online platform avoided the complicated offline procedure and band broadening. We also achieved the real-time monitoring of DNA separation during the gel electrophoresis.

Does silver stain increase sensitivity in polyacrylamide gels?

The photochemically derived silver stain of nucleic acids in polyacrylamide gels originally described by Merril et al. (1981, Science 211, 1437–1438) was modified to reduce unspecific background staining andincrease sensitivity (down to 1 pg/mm 2 band cross-section).

Does silver stain detect nucleic acids?

Heukeshoven, J. & Dernick, R. Simplified method for staining of proteins in polyacrylamide gels and the mechanism of silver staining. Electrophoresis 6, 103–112 (1985). Somerville, L.L. & Wang, K. The ultrasensitive silver 'protein' stain also detects nanograms of nucleic acids.

Can gel electrophoresis detect high-resolution DNA separation?

We also achieved the real-time monitoring of DNA separation during the gel electrophoresis. A 32–64 % increase in the number of theoretical plates suggested high-resolution DNA detection. Polyacrylamide gel electrophoresis (PAGE) is one of the most popular techniques for the separation and detection of nucleic acids.

What are the methods for silver staining nucleic acids?

The methods for silver-staining nucleic acids employ either a histologically derived procedure that uses ammoniacal solutions of silver (1-4), or a photochemically derived reaction in which silver binds to nucleic acid bases and is then selectively reduced by 'To whom correspondence should be addressed. chemical agents or light (5-8).