Can online polyacrylamide gel electrophoresis be used for nucleic acid detection?

We developed an online polyacrylamide gel electrophoresis platform for nucleic acid detection. The online platform avoided the complicated offline procedure and band broadening. We also achieved the real-time monitoring of DNA separation during the gel electrophoresis.

What is native polyacrylamide gel electrophoresis?

Native Polyacrylamide Gel Electrophoresis (Native Page) is a protein separation and analysis process based on charge and size. Unlike other gel electrophoresis procedures, such as SDS-PAGE, Native Page does not require denaturing chemicals in the gel matrix, such as SDS (sodium dodecyl sulfate).

Why is polyacrylamide gel electrophoresis important?

1. Introduction Thanks to its beneficial properties of low-cost, and high-throughput [ 1, 2 ], polyacrylamide gel electrophoresis (PAGE) plays an important role in nucleic acid separation , , and oligonucleotide detection [ 6, 7 ], such as DNAzymes [ 8, 9 ], molecular probes , cell-free DNA [ 11, 12 ], and microRNA .

How to prepare polyacrylamide gel electrophoresis?

Here is a description of the preparation steps for a continuous (10%) polyacrylamide gel for Native and Blue Native gel electrophoresis: Start by thoroughly cleaning and drying the glass plates, suitable spacers, and comb. Assemble the glass plates, spacers, and comb according to the manufacturer’s instructions.

Can gel electrophoresis detect high-resolution DNA separation?

We also achieved the real-time monitoring of DNA separation during the gel electrophoresis. A 32–64 % increase in the number of theoretical plates suggested high-resolution DNA detection. Polyacrylamide gel electrophoresis (PAGE) is one of the most popular techniques for the separation and detection of nucleic acids.

What is blue native polyacrylamide gel electrophoresis (BN-PAGE)?

Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) is a popular approach for characterizing proteins in their enzymatically active state because it provides high-resolution separation. Using Coomassie Blue-G250 dye, protein complexes are observed and given an external negative charge in BN-PAGE.