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Anionic polyacrylamides are co-polymers of acrylamide with increasing proportions of acrylate groups, which give the polymers negative charges, and thus anionic active character, in aqueous solution.

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Ammonium persulfate is used as a catalyst for the copolymerization of acrylamide and bisacrylamide gels. The polymerization reaction is driven by free radicals generated by an oxido-reduction reaction in which a diamine (e.g., TEMED) is used as the adjunct catalyst. Dissolve 10 g of SDS in 80 mL of H2O, and then add H2O to 100 mL.

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