What is polyacrylamide gel electropho-Resis (page)?

This unit describes methods and protocols for denaturing polyacrylamide gel electropho-resis (PAGE) for the analysis and purification of synthetic oligonucleotides.

Which acrylamide kits are available for hand casting polyacrylamide gels?

Acrylamide powders, premixed acrylamide/bis powders, and premixed acrylamide/bis solutions are offered for handcasting polyacrylamide gels. Standard and alternative cross-linkers and catalysts are available. TGX FastCast acrylamide kits for hand casting polyacrylamide gels enable fast, easy casting and produce long-life gels.

What is a gel based electrophoresis system?

Gel-based electrophoresis system that uses different buffers and sometimes different buffer compositions to focus and separate components of a sample. Discontinuous systems typically focus the proteins into tighter bands than continuous gel systems, allowing larger protein loads

What is the best stain for detecting proteins in polyacrylamide gels?

Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9, 255–262. Oakley BR et al. (1980). A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem 105, 361–363.

What is a cross-linked polyacrylamide gel matrix?

The cross-linked polyacrylamide gel matrix is held between two glass plates and exposed on the top and bottom to chambers containing buffered electrolyte solution through which an electric field of controlled voltage or current is applied.

Can polyacrylamide be used for protein separation?

For protein separation, virtually all methods use polyacrylamide as an anticonvective, sieving matrix covering a protein size range of 5–250 kD. Some less common applications such as immunoelectrophoresis and the separation of large proteins or protein complexes >300 kD rely on the larger pore sizes of agarose gels.