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*Corresponding author: E-mail: raid@riyadh.edu.sa; Chemical Science International Journal 25(4): 1-8, 2018; Article no.CSIJ.46483 ISSN: 2456-706X (Past name: American Chemical Science Journal, Past ISSN: 2249-0205) Synthetic Polyelectrolytes Based on

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Polyacrylamide is a high molecular weight polymer formed from acrylamide subunits. It is a white powder or granular substance with a molecular weight ranging from 5,000,000 to 30,000,000 and a degree of hydrolysis between 6-45%. Polyacrylamide is easily soluble in cold water upon rapid mechanical stirring, forming a transparent adhesive solution.

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中文名:聚丙烯酰胺,英文名:Poly(acrylamide),CAS:9003-05-8,聚丙烯酰胺是重要的水溶性聚合物，而且兼具絮凝性、增稠性、耐剪切性、降阻性、分散性等宝贵性能。

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Nonreducing 2-D Polyacrylamide Gel Electrophoresis

Apply the equilibrated IPG gel strips to 2-D 12% polyacrylamide gels (160×160×1 mm gel size) and electrophorese the samples at 25 C for 16 h using Laemmli SDS running buffer until the tracking dye reaches 1 cm from the bottom of the separation gel.

[8] Two-dimensional polyacrylamide gel electrophoresis

Görg, A., Boguth, G., Obermaier, C., Posch, A., and Weiss, W. (1995) Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt): the state of the art and the controversy of vertical versus horizontal

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Apply the equilibrated IPG gel strips to 2-D 12% polyacrylamide gels (160×160×1 mm gel size) and electrophorese the samples at 25 C for 16 h using Laemmli SDS running buffer until the tracking dye reaches 1 cm from the bottom of the separation gel.

[8] Two-dimensional polyacrylamide gel electrophoresis

Görg, A., Boguth, G., Obermaier, C., Posch, A., and Weiss, W. (1995) Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt): the state of the art and the controversy of vertical versus horizontal

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Apply the equilibrated IPG gel strips to 2-D 12% polyacrylamide gels (160×160×1 mm gel size) and electrophorese the samples at 25 C for 16 h using Laemmli SDS running buffer until the tracking dye reaches 1 cm from the bottom of the separation gel.

[8] Two-dimensional polyacrylamide gel electrophoresis

Görg, A., Boguth, G., Obermaier, C., Posch, A., and Weiss, W. (1995) Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt): the state of the art and the controversy of vertical versus horizontal

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Apply the equilibrated IPG gel strips to 2-D 12% polyacrylamide gels (160×160×1 mm gel size) and electrophorese the samples at 25 C for 16 h using Laemmli SDS running buffer until the tracking dye reaches 1 cm from the bottom of the separation gel.

[8] Two-dimensional polyacrylamide gel electrophoresis

Görg, A., Boguth, G., Obermaier, C., Posch, A., and Weiss, W. (1995) Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt): the state of the art and the controversy of vertical versus horizontal

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[8] Two-dimensional polyacrylamide gel electrophoresis

Görg, A., Boguth, G., Obermaier, C., Posch, A., and Weiss, W. (1995) Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt): the state of the art and the controversy of vertical versus horizontal

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Anionic polyacrylamide has a negative charge due to the presence of anionic groups (such as carboxylate or sulfate groups), while cationic polyacrylamide has a positive charge resulting from cationic groups (such as amino or quaternary ammonium groups).

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