Is polyacrylamide-coated silica a stationary phase for hydrophilic interaction liquid chromatography (HILIC)?

A polyacrylamide (PAM)-coated silica packing as a novel stationary phase for hydrophilic interaction liquid chromatography (HILIC) was produced firstly by thermal immobilization.

Is polyacrylamide coated silica hydrophilic?

Although there are no chemical bonds involved, the bleeding measurement and the background noise are acceptable. Polyacrylamide coated silica (PAM-Sil, mean diameter: 5 μm) demonstrated a good hydrophilic property and excellent separation efficiency, which was observed especially for several high polar analytes such as saccharides.

Is polyacrylamide a hydrophilic polymer?

Polyacrylamide (PAM) is a highly hydrophilic polymer with a generally atactic structure . Hence it was previously modified onto the monolithic silica capillary columns for the separation of polar compounds by Ikegami and Debs [17,18].

Are acrylamide-based monolithic stationary phases efficient for HILIC separation?

Acrylamide-based monolithic stationary phases were successfully created within 200 μm ID capillaries using thermal polymerization. From SEM pictures, the microglobular structure was seen to be homogeneous and well bounded to the capillary wall. These highly hydrophilic monoliths were optimized for efficient HILIC separations of intact proteins.

Can alcohol be used as a weak eluent in hydrophilic interaction liquid chromatography?

A polyacrylamide-based silica stationary phase for the separation of carbohydrates using alcohols as the weak eluent in hydrophilic interaction liquid chromatography J. Chromatogr. A, 1524(2017), pp. 153-159

Can a polymerization mixture be used for hydrophilic-interaction chromatography of intact proteins?

In this study, we optimized a polymerization mixture to synthesize poly (acrylamide- co – N, N ′-methylenebisacrylamide) monolithic stationary phases for hydrophilic-interaction chromatography (HILIC) of intact proteins.