Can polyacrylamide gel electrophoresis be used for protein staining?

The present review concentrates on techniques for the staining and quantification of proteins separated by polyacrylamide gel electrophoresis. Staining with organic dyes has been used for approximately thirty years; the silver staining technique was introduced in 1979.

Is polyacrylamide gel electrophoresis a quantitative method?

Polyacrylamide gel electrophoresis is widely used for studying proteins and protein-containing objects. However, it is employed most frequently as a qualitative method rather than a quantitative one. This paper shows the feasibility of routine …

What is intrinsic fluorescence detection in polyacrylamide gel electrophoresis (PAGE)?

Thanks to its label-free and stain-free feature, intrinsic fluorescence detection has been introduced to polyacrylamide gel electrophoresis (PAGE), a fundamental and ubiquitous protein analysis technique, to avoid the tedious detection process.

What is protein electrophoresis in a gel matrix?

Electrophoresis in a gel matrix stands as the most popular technique, employing a variety of gel materials such as starch gels, dextran gels, agarose gels, and polyacrylamide gels . In this protocol, we will focus on protein electrophoresis in polyacrylamide gels.

Is there an online intrinsic fluorescence imaging method for stain-free polyacrylamide gel electrophoresis?

We developed an online intrinsic fluorescence imaging method for stain-free polyacrylamide gel electrophoresis. We redesigned the arrangement of the light source and the gel electrophoresis apparatus for high imaging performance. Online imaging realized the label-free and real-time monitoring of the protein separation process.

Can aprotinin be analyzed by gel electrophoresis in polyacrylamide gel?

Meanwhile, as aprotinin derivatives have a protein nature, they can be analyzed by polyacrylamide gel electrophoresis. The target aprotinin and its impurities can be detected by gel electrophoresis as clearly as by chromatography . Thus, the methods of HPLC and electrophoresis in polyacrylamide gel can be interchanged.