Can online polyacrylamide gel electrophoresis be used for nucleic acid detection?

We developed an online polyacrylamide gel electrophoresis platform for nucleic acid detection. The online platform avoided the complicated offline procedure and band broadening. We also achieved the real-time monitoring of DNA separation during the gel electrophoresis.

What is intrinsic fluorescence detection in polyacrylamide gel electrophoresis (PAGE)?

Thanks to its label-free and stain-free feature, intrinsic fluorescence detection has been introduced to polyacrylamide gel electrophoresis (PAGE), a fundamental and ubiquitous protein analysis technique, to avoid the tedious detection process.

What is polyacrylamide gel electrophoresis (PAGE)?

As a well-established electrophoresis technique, polyacrylamide gel electrophoresis (PAGE) is ubiquitously applied to routine protein analyses in biochemistry and molecular biology labs [, , ].

What are acrylamide electrophoresis gels used for?

Acrylamide electrophoresis gels are used for the separation and analysis of nucleic acids and proteins. Products include precast gels designed for analysis of specific biomolecules and welcome packs containing gels, buffers, and reagents.

Is there an online intrinsic fluorescence imaging method for stain-free polyacrylamide gel electrophoresis?

We developed an online intrinsic fluorescence imaging method for stain-free polyacrylamide gel electrophoresis. We redesigned the arrangement of the light source and the gel electrophoresis apparatus for high imaging performance. Online imaging realized the label-free and real-time monitoring of the protein separation process.

Can gel electrophoresis detect high-resolution DNA separation?

We also achieved the real-time monitoring of DNA separation during the gel electrophoresis. A 32–64 % increase in the number of theoretical plates suggested high-resolution DNA detection. Polyacrylamide gel electrophoresis (PAGE) is one of the most popular techniques for the separation and detection of nucleic acids.