What is a UV transilluminator used for?

The main usage of a UV transilluminator is in the visualization of protein and DNA polyacrylamide and agarose gels after the process of electrophoresis. It provides researchers with the compact footprint of the gel. The gels are placed directly on the UV transilluminator. But the wavelength will change depending on the specific application.

Can gels be placed on a UV transilluminator?

Gels can be directly placed onto the UV transilluminator; wavelength will vary on your particular application. Some considerations to keep in mind when shopping around for a UV transilluminator include viewing surface size for varying size gels; wavelengths, as some models come in single or dual wavelengths; and the dimensions of the benchtop unit.

What are UV-transilluminators used for?

UV-transilluminators •UV-transilluminators are used in molecular biology labs to view DNA (or RNA) that has been separated by electrophoresis through an agarose gel. •Samples are placed on the illumination Ultraviolet Radiation 265 platform and are illuminated by the UV light

Why do we need a UV absorbing cover in a transilluminator?

Since intense UV light is used for visualization of fluorescent markers used in agarose gel electrophoresis, the radiation is perilous to both eyes and skin. Hence, UV absorbing cover must be fitted in the transilluminator.

How does a transilluminator work?

A transilluminator works by emitting high-intensity UV radiation through the surface of the object being viewed. Specific wavelengths of UV or visible light are emitted onto a glass viewing surface (where agarose or polyacrylamide gels are placed). The transilluminator can be used as a stand-alone system or in conjunction with a gel imager.

What wavelength is a UV transilluminator used for?

Depending on the type and amount of sample, the UV transilluminator is operated in any of the three wavelength bands. 254, 312, and 365 nm are typical wavelengths. 312 nm are used for standard gel documentation in the gel doc system.