How are methylene blue loaded PLGA nanoparticles prepared?

In this study, methylene blue (MB) loaded PLGA nanoparticles (MB-PNP) are prepared by a new approach combining single and double emulsification techniques. Comparisons of MB-PNP obtained with the combined and the individual techniques are presented.

Can methylene blue be covalently encapsulated in amine-modified MSNs?

6. Conclusion In summary, this study introduces a one-pot synthesis approach for covalently encapsulating methylene blue (MB) in amine-modified MSNs, addressing challenges associated with conventional encapsulation methods.

How to prevent methylene blue from being reduced by plasma reductases?

The ability to prevent methylene blue (MB), a photosensitizer, from being reduced by plasma reductases will greatly improve its efficacy in photodynamic therapy (PDT) applications. We have developed a delivery approach for PDT by encapsulating MB using nanoparticle platforms (NPs).

What is methylene blue (MB)?

Methylene blue (MB) is a fluorescent dye that has an excitation wavelength (λ ex) of 660 nm and emission wavelength (λ em) at 680 nm, which falls in the NIR region. It is an FDA approved drug for methemoglobinemia . Moreover, the photosensitizing potential of MB makes it a promising agent for PDT .

Is methylene blue a cationic dye?

Methylene blue (MB) is a cationic dye with an ability to act as photosensitizing and bioimaging agent. The direct utilization of MB as photosensitizer for biological applications has often been impeded by its poor photostability and unwanted tissue interactions.

How big is MB-PNP compared to double emulsion?

Particles prepared by the combined technique presented hydrodynamic diameters of 186 nm. The sizes of MB-PNP obtained from the single emulsion technique are similar to the combined technique, while the diameter of particles prepared by double emulsion increased from 201 nm to 287 nm as the TDL increased.