Can I extract DNA from polyacrylamide gels?

The QIAEX II and QIAquick Gel Extraction Kit can be used to extract DNA from polyacrylamide gels. The QIAEX II Handbook contains a protocol for Polyacrylamide Gel Extraction. A specialized User-Developed Protocol (QQ05) is available when using the QIAquick Gel Extraction Kit for this purpose.

What is a gel/PCR DNA fragment extraction kit?

The Gel/PCR DNA Fragments Extraction Kit was designed to recover or concentrate DNA fragments (70 bp-20 kb) from agarose gel, PCR, or other enzymatic reactions in one convenient product. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in chaotropic salt are bound by the glass fiber matrix of the spin column (1).

Can Guanidinium chloride interfere with DNA sequencing?

Please use this protocol for sequencing as Guanidinium Chloride, a component of W1 Buffer, may interfere with sequencing reactions. Excise the agarose gel slice containing relevant DNA fragments and remove any extra agarose. NOTE: Using TAE buffer for gel formation is recommended for optimal DNA recovery.

What percentage of eluted DNA can be used for PCR?

Typically, recoveries are up to 90% for Gel Extraction and up to 95% for PCR Clean Up. The eluted DNA is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation.

How do you remove contaminants from DNA?

Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer or TE. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture, without phenol extraction or alcohol precipitation.

What is the best elution buffer for a DF column?

If DNA fragments are larger than 8 kb, use pre-heated Elution Buffer (60-70 oC) to improve the elution efficiency. Dry the DF Column with additional centrifugation at 14-16,000 x g for 5 minutes or incubate at 60 oC for 5 minutes.