How is vitamin B 12 analyzed?

Traditionally, vitamin B 12 is analyzed by a non-specific and time-consuming microbiological assay that uses Lactobacillus leichmannii (ATCC 7830) as the test organism (Kumar, Chouhan, & Thakur, 2010).

Is 228 NM a good wavelength for vitamin B 12 cobalamins?

Compared to 360 nm which is a popular wavelength that AOAC official method 2014.02 used (Giménez & Martin, 2018), 228 nm provided a better peak resolution for all four vitamin B 12 cobalamins especially when approaching the low concentration in samples (<5 ppm).

What is the AOAC official method for determining total vitamin B 12?

AOAC official method 2014.02 is efficient in determining total vitamin B 12 using common instrumentation (HPLC-UV). However, this method converts all forms of vitamin B 12 into cyanocobalamin and therefore does not distinguish the source of them (Giménez & Martin, 2018).

How many bioactive vitamin B12s are derived from dietary supplements?

Simultaneous determination of four bioactive vitamin B12s from dietary supplements. The method is simpler, less time-consuming, and more economical than other methods. The LOQs are less than 0.52 µg/mL. The method is validated and meets all AOAC SMPR 2016.017 requirements.

What is the LOQ of vitamin B12 dietary supplement?

The LOQs are less than 0.52 µg/mL. The method is validated and meets all AOAC SMPR 2016.017 requirements. Vitamin B12 dietary supplement can be critical to the alleviation strategies against micronutrient malnutrition and food insecurity.

Is vitamin B 12 a good nutrient?

To our best knowledge, it is simpler, less time-consuming, and more economical than other published methods for its intended uses. Vitamin B 12 (cobalamin) is a water-soluble micronutrient that functions as a coenzyme of several key enzymes in one-carbon (methyl) metabolism and is essential for human growth and development (Selhub, 2002).