What are Tricine gels?

The Tricine Gels are based on the Tricine system developed by (Schaegger and vonJagow, 1987). In this buffer system, tricine substitutes glycine in the running buffer resulting in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.

What is a discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis system?

A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described. Tricine, used as the trailing ion, allows a resolution of small proteins at lower acrylamide concentrations than in glycine-SDS-PAGE systems.

What is Tricine SDS-PAGE used for?

Nature Protocols 1, 16–22 (2006) Cite this article Tricine–SDS-PAGE is commonly used to separate proteins in the mass range 1–100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in other electrophoretic systems.

Are Tricine gels better than Tris-glycine?

The Tricine Gels have the following advantages over the Tris-Glycine Gels for resolving proteins in the molecular weight range of 2-20 kDa: The Tricine system is a modification of the Tris-Glycine discontinuous buffer system specifically designed for the resolution of low molecular weight proteins.

What is acrylamide Tricine – SDS?

Modified, with permission, from ref. 2. Uniform acrylamide Tricine–SDS gels cover narrow mass ranges—for example, 10% gels cover the range 1–100 kDa (Fig. 2a) and 16% gels cover the range 1–70 kDa (Fig. 2b)—and offer high resolution, especially for the small protein and peptide range.

Which acrylamide gel should be used for Tricine–SDS-PAGE?

Using low-acrylamide gels for Tricine–SDS-PAGE (for example, 10% acrylamide gels) is advantageous for rapid separation, for covering a relatively wide mass range (2–100 kDa) and for efficient electroblotting and electroelution.